Fourth Methods in Cytokine Biology Minisymposium


Tuesday January 28th, 2003

8:30 AM-3:30 PM

Natcher Conference Center, NIH Campus

No registration or fee required

Photo ID required for building entry

Meeting Chairs: Calman Prussin/ NIAID, Ron Rabin/CBER

Plenary Session

9:00-12:15 Natcher Auditorium

Time

Topic

Speaker

Affiliation

8:30

Proteinomic Analysis of Cytokine Responses

Francesca Aiello

NCI

9:10

TGF-b Quantitation

Wanjun Chen

NIDCR

9:40

Electrochemiluminescence Assays

George Sigal

Meso Scale Discovery
Coffee Break

10:30

Cytokine Protein Arrays

Ruo-Pan Huang

Emory University

11:00

Multi-analyte Cytometric Bead Assay

Kathryn Kellar

CDC

11:30

Intracellular Cytokine Staining

Steven De Rosa & Jason Brenchley

NIAID

12:00-1:00 PM Sandwich lunch provided - Vendor booths open all day

Investigator Led Workshops

Concurrent sessions, 30 min each
1:00-1:30 pm
1:40-2:10 pm

Steven Migueles/ NIAID; Stephen Haley/ Beckman Coulter

Using MHC Tetramers to Investigate Cytokine Responses

Paul Lehman/ Case Western Reserve

ELISPOT

Ligia Pinto/NCI

Validating Cytokine Bead Assays in a Clinical Trials Setting

Kathryn Kellar/ CDC

Multianalyte Cytometric Bead Assay

Bill Kopp/ NCI

Can Serum Cytokines be Meaningfully Measured?

Howard Young/NCI

Multiprobe RNAse Protection Assay

Jason Brenchly/ NIAID

Intracellular Cytokine Staining

Chang-you Wu/NIAID

MACS Cytokine Secretion Assay

Vendor Tutorials

Concurrent sessions, 30 min each
2:20-2:50 pm
3:00-3:30 pm

Linco Research, Inc.

Multiplex Analyses of Cytokines, Chemokines and Endocrine Hormones

Biacore

Biacore Principles and Applications in the Study of Cytokines

R&D Systems

Fluorokine MAP: Cytokine Profiling Kits for the Luminex Platform

Meso Scale Discovery

Electrochemiluminescence Based Cytokine Assays

Millipore Corp.

Microplate and Membrane Technology for the Elispot Assay

The ELISPOT Resource Group

New Tools for the Simplification and Standardization of the ELISPOT Technique

Miltenyi Biotec

Isolation and Analysis of Viable and Functional Antigen-Specific Immune Cells

BD Biosciences

Cytometric Bead Array, Granzyme B Elispot Assays, BD FACS Array Bioanalyzer

Pierce-Endogen

SearchLight Proteome Arrays

Perkin-Elmer

Practical Applications of Protein Microarrays

Open to NIH and outside investigators

Campus Map Directions

Email info: calman@nih.gov

www: http://www.nih.gov/sigs/cytokine

Sponsored by the NIH Cytokine Interest Group (CIG) with generous support from these additional meeting sponsors:

Beckman-Coulter, Biorad, Biosource, Cell Techology Inc., C.T.L.-Cellular Technology Ltd., Caltag, Invitrogen, One Cell Systems, Peprotech, Polysciences, Sanyo, Schleicher & Scheull Bioscience